This short marks the debut appearance of Tootie, though here, she was voiced by Amber Hood, rather than Grey DeLisle, In this episode, Tootie is already madly in love with Timmy. Tootie's glasses have round frames and she wears different clothing. Her pig-tails are shorter, and her hairline is different. Her pupils are blue instead of black.
In addition to this being the debut episode of Tootie, this is also the first time we see Timmy with other kids which is a fairly rare occurrence in the Oh Yeah! Cartoon shorts.
Cosmo sneezed exactly seven times in this episode, while Wanda sneezes four times.
This is the first time that an episode title card is animated.
This is the first time an on-screen death is shown in the franchise (in this case, the butterfly being killed by a speeding truck.)
First episode where Timmy gets kissed by someone (Tootie, in this case).
This is the second episode with an animated title card.
When they played spin the bottle, Tootie said that the winner gets to kiss, her, but one of the player is a girl who seemed to not care if she won.
Vicky's House is colored brown and yellow at first before turning green and white.
The clown is ridiculously tall in comparison to the rest of the house in some scenes.
In the very first episode, Vicky mentions she has a brother, but no other member of her family is mentioned or seen besides Tootie.
Most likely the brother was dropped and replaced with Tootie, as this was only referenced in the pilot episode.
Towards the beginning of the episode, Timmy does not want to go to the party and explains to Cosmo and Wanda that his parents would say, "It would be a nice gesture if you [he] go[es]." He opens the door and they say that. Cosmo and Wanda are in their normal form, which means Timmy's parents should have seen Cosmo and Wanda.
In this episode, the Fairy Flu makes fairies temporarily loose control of their powers. In Timmy the Barbarian!, Binky gets the flu, but he does not uncontrollably poof things up when he sneezes.
Timmy: A birthday party? Ah, rats! [Sees Cosmo and Wanda being rats] GAH!!!
Tootie: [Scared of Timmy, because his head is a toilet] Gah!!
Clown: [sarcastically] "No mom, I don't need college, I gonna 'follow my dream' and become a clown."
Vicky: Hello there, I'm Vicky, and I'm the... ABSOLUTE RULER!
Boy: How come you're the absolute ruler? [Other party guests back away]
Vicky: Because, my little artichoke, I'm 16 and you're 10. DO THE MATH!!!!
Vicky: These are the [birthday] party rules. Now I am only gonna say this once, so pay attention! Now then... [Cosmo sneezes and she turns into a frog] Ribbit ribbit ribbit ribbit... [catches a fly with her tongue] Ribbit ribbit ribbit ribbit ribbit!