Ironically, at the beginning of the episode (after the theme song) the theme song was shown before the flashback of the previous episode was shown.
In the original script of this episode, it was titled A World of Heroes, and Bull-E was called Fran-Thing. Hawk Gal also had the power to talk to birds, although only succeeds in making one poop on her head. Other heroes like Wonder Gal and the Sonic Youth also had slightly larger roles.
Apparently when the Nega-Chin was about to be fully sealed back into the comic book he sent Short Fuse into the real world to keep an eye out for anything that would turn the real world into a comic book world and notify him if something like that would happen.
Got Milk? - when Vicky says "Got Milk?- on your head!". This is a reference to the commercials.
Zork II: The Wizard of Frobozz - Doctor Crocktopus attacks that begin with the "F" is a reference to the Wizard cast spells that begin with the "F". Also, at the end, when Nega-Chin inadvertently frees Ace and Clefto, the sound effect that is written on the cloud is "ZORK!".
Butch Hartman - "Elmer Marmelman" is a pun off of the show's creator's real name.
People noting on Veronica not really being a great superhero.
Veronica's pupils are pink in this episode when they are normally blue.
Soon after the fake Crimson Chin (Nega-Chin) did his evil laugh, Timmy/Cleft can be seen briefly with no buck teeth when his mouth is closed.
When Timmy screams after the Nega Chin makes the wish, he has regular teeth instead of his trademark buckteeth.
During the live action sequence, when the Crimson Chin Author opens up the door, Nega-Chin is seen in an animated background and not a live action background.
When Timmy unintentionally wished for a world without superheros, Cosmo and Wanda as Clefto and Ace did not seem to be affected. This may be because they are fairies, and fairies are not exactly "superheroes". And also, In Mighty Mom and Dyno Dad, it says that magic cannot affect superheroes, but in this episode, it is unknown and unexplained why Crimson Chin and the rest of the superhero alter-egos of Timmy and the gang was affected by the wish.
When Dr. Crocktopus finishes a certain quote with "and fix them for good!", the captions instead finish it with "that fixed them for good!". It's as if Dr. Crocktopus has already fixed them, when he actually hasn't.